![]() KEY POINTS: An increase in malate level of GID4 mutant resulted from the accumulation of Mdh2. Our results suggest that high-malate-producing disruptants adapt their metabolism to produce malate in excess via the regulation of protein expression in glucose assimilation and ethanol fermentation. The high-malate-producing disruptants showed the activation of several glycolytic enzymes and a reduction in enzymes involved in the conversion of pyruvate to ethanol. Comparison of protein abundance among the GID disruptants revealed variations in protein expression profiles, including in glycolysis and tricarboxylic acid cycle–related enzymes. The disruptants of GID1, 2, 3, 4, 5, 8, and 9 exhibited high malate production. ![]() Next, we investigated the effects of GID protein–coding genes (GID1–GID9) on organic acid production and enzyme expression profiles in yeast. Furthermore, target proteome analysis indicated that an increase in malate production resulted from the accumulation of Mdh2 in gid4 disruptant due to the loss of GID complex–mediated degradation. The evaluation of disruptants and mutants of gluconeogenic enzymes revealed that cytosolic malate dehydrogenase (Mdh2) participated in the malate production. In this study, the mechanism by which this mutation led to high malate production in yeast cells was investigated. Gid4 is a component of the GID (glucose-induced degradation-deficient) complex and stimulates the catabolic degradation of gluconeogenic enzymes. We previously isolated a high-malate-producing strain and found that a missense mutation in GID4 was responsible for the high-malate-producing phenotype. Since malate contributes to the pleasant flavor of sake (a Japanese alcoholic beverage), various methods for breeding high-malate-producing yeast have been developed. 4971-4983 ISSN: 0175-7598 Subject: Saccharomyces cerevisiae, acetates, biotechnology, ethanol, ethanol fermentation, flavor, glucose, glycolysis, malate dehydrogenase, malates, missense mutation, mutants, phenotype, protein synthesis, proteome, pyruvic acid, sake, succinic acid, yeasts Abstract: During alcohol fermentation, Saccharomyces cerevisiae produces organic acids, including succinate, acetate, and malate. Effects of mutations of GID protein–coding genes on malate production and enzyme expression profiles in Saccharomyces cerevisiae Author: Hiroaki Negoro, Kengo Matsumura, Fumio Matsuda, Hiroshi Shimizu, Yoji Hata, Hiroki Ishida Source: Applied microbiology and biotechnology 2020 v.104 no.11 pp.
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![]() 8 ' High 'n' Dry (Saturday Night)', ranked No. High 'n' Dry was Pete Willis ' last full-time album with Def Leppard. Multiple studios in London, one guitarist out, one guitarist in, equipment breakdowns, tapes turning transparent because of the thousands of times they were rewound & fast forwarded for multiple overdubs, but we found our sound on this record with the help of a great producer in Mutt Lange, the new studio technology that we eagerly embraced (unlike many of our peers) and an incredible enthusiasm to "make a record no one else had ever made" whether we did or didn't isn't important, what is, is that we had made the record WE wanted to make, it had been percolating in our DNA for 6 years!!…. High 'n' Dry is the second studio album by the English rock band Def Leppard, released on 6 July 1981. Setlist Rock Rock (Till You Drop) Rock Brigade High & Dry (Saturday Night) Another Hit and Run Billys Got a Gun Foolin Photograph Rock of Ages. I can't believe it's 30 years since Pyromania came out! It had been a labour of love to make (little did we know in comparison to Hysteria, it was a piece of cake!). we finally sounded like the "us" we wanted to be!!! - J x Multiple studios in London, one guitarist out, one guitarist in, equipment breakdowns, tapes turning transparent because of the thousands of times they were rewound & fast forwarded for multiple overdubs, but we found our sound on this record with the help of a great producer in Mutt Lange, the new studio technology that we eagerly embraced (unlike many of our peers) and an incredible enthusiasm to "make a record no one else had ever made" whether we did or didn't isn't important, what is, is that we had made the record WE wanted to make, it had been percolating in our DNA for 6 years!!…. I can't believe it's 30 years since Pyromania came out! It had been a labour of love to make (little did we know in comparison to Hysteria, it was a piece of cake!). Def Leppard have sold more than 110 million albums worldwide, including two of the best-selling albums of all time, Pyromania and Hysteria. Here is a special note from Joe Elliott on the album. Today marks the 30th anniversary of the release of DL'S album PYROMANIA. Celebrating PYROMANIA - track by track Visit The LEPPARD VAULT for song analyses and stories from each band member EXPLORE at LISTEN TO DEF LEPPARD. From start to finish, it's a flawless, iconic rock masterpiece IMHO, this was the album that truly influenced everything after it. The album was a HUGE smash, selling more than 10 million copies in the US alone, and it was certified diamond (eventually Def Leppard would gain another diamond record for 'Hysteria', making them one of the only 5 bands in history to achieve that mark!) Def Leppard released 'Pyromania' and they became legends. ![]() What’s all the buzz about? 25% of monthly matches went on dates.īumble seems to be a favorite among people who are looking for something serious. ![]() Whereas people older than 50 account for more than 45% of the FirstMet and Christian Mingle user bases. In fact, the average age on Bumble and Tinder is 26.įor comparison, the majority of people who use OkCupid, Coffee Meets Bagel, or Grindr fall in the 30-49 age group. Tinder follows a similar trend: the younger the users, the more likely they are to use the app. The latest Bumble stats suggest that 63.3% of users are younger than 30, whereas less than 10% of swipers are older than 50. The odds seem to be in Bumble’s favor as it tops the charts for the youngster group, which incidentally happens to be Bumble’s target audience. Age is just a number-Bumble strikes lucky with Millennials. And the latest stats show the trend remains-32.6% females against 67.4% males in the US. Stats suggest that 52.4% of online daters are male.īut, interestingly enough, the disparity is much more dramatic if we’re talking about this app in particular-Bumble’s gender ratio is significantly skewed in favor of the male population.īack in 2019, only 34.5% of the app’s active users were female. Overall, men are more likely to use dating apps than women. Back in 2018, the number sat at only 4.5 million global MAU. This might seem like meager pickings compared to Tinder’s impressive 75 million monthly users, but Bumble’s growth is worth a second look. Around 5.5 million people in the US are active on Bumble each month.Ĭomparatively, the rest of the world contributes 6.8 million more, which means the count adds up to 12.3 million active users per month. For example, the dating app ranks #8 in North America, #11 in Germany, and #14 in the UK.īut how many people actually use Bumble? 4. Now, if we focus specifically on the Lifestyle category, Bumble doesn’t fare so bad. By the end of 2021, Bumble ranked 199th in the App Store.īumble continues to pine after a spot in the top 100 list.īoth Tinder and Bumble fell down the App Store’s general rankings in late 2021, but since 2022 started, Bumble seems to be slowly buzzing its way back up the list. That said, Bumble statistics reveal that 56.61% of downloads come from the Americas. Naturally, Tinder rules 86% of them all, whereas Badoo comes in second place with 7%. What we do know is that Bumble tops the ranks in a mere 2% of all countries. There are no public clues as to where its users abide. The geographic expansion of Bumble can best be described as uncharted territory. Bumble apps are accessible in 150+ countries. Locking Bumble and Badoo numbers together brings about 600 million users in total. In 2020, Bumble’s user base grew to 100 million.īy the end of 2015-that is, one year after it launched-there were only one million active Bumble users, which makes the jump to 100 million all the more impressive.Īnd more so if we take into account both Bumble Inc. Let’s check out what Bumble stats have to say. So, have women stolen men’s thunder in the online dating world? If a woman does not chat up the person she matched with within 24 hours, the match automatically terminates. In a world where sexual harassment and seems to be around every corner, it’s no wonder many women were hessitant to sign up for dating apps.īut Tinder’s ex-marketing president, Whitney Herd, found a solution and transformed it into a new dating app.īumble offers the only date space where women are the sole conversation-starters in a heterosexual match.
![]() VPNs unblock this content by masking your IP address and making it seem like your traffic is coming from an unrestricted region. Many websites and streaming services like Netflix, Hulu, or Amazon Prime restrict access to content based on user locations. Giving you access to geo-blocked websites and content. ![]() ![]() VPNs can also hide your personal IP address from online apps and services that collect data. Even internet service providers (ISPs) won't see the specifics of this online activity. VPNs disguise your online activity by connecting you to a remote server and encrypting your internet traffic. Keeping your browsing activity private.Some organizations make a VPN connection mandatory for remote workers. VPN servers prove essential when you’re traveling or not on your home network. Public Wi-Fi networks are notoriously dangerous. Protecting your data while traveling or working remotely.Even if hackers are on the same network as you, they won’t see your IP address, location, or any data that you send online. VPNs use strong levels of encryption to hide your sensitive data from onlookers. Hiding your IP, location, and data from hackers.Some of the main reasons you'll want to use a VPN include: It also makes VPNs essential tools for helping to prevent identity theft and IP address hacking. This means that both internet service providers (ISPs) and individuals (such as hackers) won’t be able to track your browsing history or see your data. What Is a Virtual Private Network (VPN)? Why Do You Need One?Ī VPN reroutes your IP address and activity to an encrypted remote server where your personal information and browsing data get scrambled and concealed. paid VPN services, and outline what you get for your subscription fee. In this guide, we'll take a look at what VPNs do, compare free VPN vs. While paying for a premium VPN won't solve all your problems, it's a step in the right direction. Just one year earlier, data from another 21 million VPN users leaked and went up for sale. Sadly, this is far from the only free VPN service data breach. In 2022, researchers discovered over 25 million user records from BeanVPN had leaked on the Dark Web. Virtual private networks (VPNs) promise to help you surf the internet anonymously, access location-specific content and streaming platforms, and protect your data from snooping hackers.īut for the thousands of people using the free BeanVPN service, this unfortunately wasn't the case. At the moment the ones which will not be moving are backup, restore, and the plugin installer. We cannot move all operations, because some of them need state to persist between page loads. Reads are much faster though.Īs a side note, we are moving many of the operations which happen in tempdir to localcachedir where possible. The other thing to bear in mind is that RAID1 is typically slower than without RAID because you have to write to both disks and have confirmation that both disks wrote successfully before the operation completes. As a result it may be slower than if you are running in a single machine system. The test above is a write test of the tempdir, which is one of those directories which must be on a shared file system. The thing about shared file systems is that they are typically slower - they have to handle file locking, and are usually network based file-systems (which are also typically slower). The localcachedir is a local option which does not have to be shared. Of these, the dataroot, tempdir, and cachedir must be shared between all nodes in your cluster. These include the ability to set your dataroot, as well as tempdir, cachedir, and localcachedir. Moodle has several directory options available to you to help you with performance. There are a couple of things to bear in mind here. If you are worried about a tmpfs for your moodle tempdir being too small consider adding an SSD to your setup for the purpose instead, ramfs does not have space restrictions and will grow as large as free memory will allow will then spill over to swap causing a huge hit to your IO performance. but be aware of it and manage it if needs be.Īn alternative to tmpfs is ramfs. For you you have such a totally ridiculous amount of RAM this probably won't be an issue. If the backup process runs out of space it fails and can leave partial files lingering in your tmpfs taking up space causing knock on problems. This can need GBs of data depending on your course. Moodle uses it's tmpdir for all sorts of things, including backup and restore of courses. This is only used when data is stored, space allocated to tmpfs is available to the OS for other uses when it is not being used by the disk. Tmpfs by default allocates a size of 50% RAM to the drive. Yes, you do need to be aware of some things here. Google is your friend if you need help with any of the specifics here. Now this works your'll want to add these mountpoints to your /etc/fstab so that they come up automatically on reboots. Purge your moodle caches in the usual fashion and re-run the benchmark. you have bucket loads of RAM so I'm choosing not to worry about sizing here. Then mount a 'ramdisk' to this these locations, e.g.Īnd then again for your cache and localcache dirs. ![]() As we are only talking about Temp and Cache data we can just put this all into memory rather than writing to disk at all (this has the advantage of freeing up some IO for things that do actually need it too)įind out where you temp and cache dirs are (usually under moodledata, but also check your config.php to see if they have been manually set elsewhere). As you only have one web server you don't need to worry about sharing these folders between nodes so ignore any discussion about that. The short solution for you is to point moodles temp and cache directories to a faster storage medium. You have an issue with the write speed to some of your data locations, and specifically this test is showing results for tempdir. Should I take some ram and turn it into a RAMdisk for moodledata/temp? I'm at a loss to how to improve this though. The CPU's aren't the newest or fastest and I know that. Authentication isn't something I'm super concerned with because we authenticate through Google anyway. The write speed is what is killing me here. Last year I only had 1 physical CPU and everything was on the same VM, but I've since added a second CPU, added the 12,000 RPM SAS drive, and doubled the ram, but it "feels" "slower". I currently have a RAID 1 setup for both drives. Hard drives - webserver is on a SAS 7200 rpm enterprise server drive and the SQL server is on a 12000RPM SAS drive. The server has 2 Intel E-5 2620v2 chips - each has 6 cores, + hyperthreading 2.1ghzĮach VM has roughly half of the available resources (i.e. Because of CPU core limitations in the version of VMware we are using I can't utilize all my CPU cores with just one VM. I'm running VMware with 2 VM's - 1 for the web server, 1 for the database server. I ran this test on my production server and had some disturbing results. Yarn: Schoppel-Wolle Cashmere Queen ( Worsted Weight #4) ![]() I bet you could even wear this one as a hooded cowl! With 4 colors and 4 distinct stitch patterns, this pattern certainly isn’t boring! I like oversized cowls that can be worn down over the shoulders like a shawl as shown, or bunched up around the neck. The unique moebius cast on gives this cute accessory it’s signature twist. Join my Facebook group (lots of awesome crochet talk there – knitters welcome, too!)Īlso, you can find me on Ravelry or join more than 25K subscribers on YouTube.Ģ5+ Fabulous and Free Fall Knitting Patterns 1. Instagram (get sneak peeks of what’s coming up!)įollow me on Pinterest (I pin tons of free patterns to my boards!) I would love for you to come follow me on Make them in chunky yarn or super bulky yarn for even more squish factor!Ĭlick here to see the Easy Basketweave Knit Pumpkin Pattern! Before we begin… let’s get Social! This pumpkin knitting pattern is ready for fall! You can use the mini pumpkins on a wreath or the medium or large pumpkins for decor. 25+ Fabulous and Free Fall Knitting Patternsįeatured Pattern for Fall Knitting Patterns.Featured Pattern for Fall Knitting Patterns.You can fall-ify every room of your house – from the kitchen, to the living room, to the bedroom.Īre you ready to dig into these fall knitting patterns? Scroll down and prepare to be inspired! If you find yourself spending more time indoors this time of year, why not make a few things for around the house? We have fall knitting patterns for throws, pillows, pot holders, buntings, and more. It is so much easier to enjoy the season when you’re not freezing – especially if your new knit makes you feel extra cute and confident. We’ve got fall knitting patterns for sweaters, ear warmers, beanies, scarves and more. I know that back to school time can put folks in survival mode, but let’s choose to thrive this fall instead.įall is a great time of year to knit yourself some wearables or accessories. Fall is honestly one of the best times of year to knit, so it makes sense that there are a ton of amazing fall knitting patterns out there. When the weather gets colder you can find me inside snuggled up with a good book or my current work in progress. “I am a child of the Moon being raised by the Sun in a world walked by stars and a sky drawn with flowers.Wednesday the 22nd was the official first day of fall! Are you ready for fall yet or did it catch you off guard? I know it can be hard to say goodbye to summer, but I bet this post will help get you in the fall mood. A classic, durable, soft yarn that I had designed specifically for sweater knitting! It’s going to hold up well due to its tight twist and provide years of wearing delight! I hope that is helpful! The pattern is here if you’d like to download it.Īnd the yarn we used is our luscious (new!) Enduring Worsted. ![]() If you’d love more info on fairisle knitting, we did videos earlier in the year which you can watch here. Then switch when working the stockinette section. If that is the case, just make a note of it and adjust your needle size when working that part of the sweater. Some people find their fairisle gauge is different than their stockinette gauge. So repeat that process working one of the fairisle charts and see what you get. NOTE: I will say too, on this sweater, you may want to also check your fairisle gauge. Then begin knitting your beautiful sweater. ![]() Go down a needle size and repeat the process.ĭo this until you get the same gauge as the designer. If you’re getting too few stitches per inch, your gauge is looser than the designers. Go up a needle size and repeat the entire process. If you have too many stitches per inch, it means your gauge is tighter than the designer’s. I like to use pins and a tape measure for this.Ĭompare with the designer. Then measure your stitches per inch and rows per inch. Just like you would your finished sweater.Ĭut all those loops on the back so you can lay your swatch flat. ![]() The quicker we can get to knitting, the better, eh? You’ll have funny, big loops on the back of your work!ĭoing a swatch this way rather than alternating knit and purl rows will give you a more accurate in-the-round gauge swatch.Īlso, you could just knit a swatch fully in the round using the magic loop method, but this route saves you a smidgen of time. Repeat that process over and over until you have a nice size swatch. Pull your yarn loosely behind the knitting and knit the next row. Now, slide your knitting to the opposite needle. You’ll need circular needles – you’ll want to start with the needle size the designer recommends and the yarn you intend to use.Ĭast on a few dozen stitches or so and knit the first row. To knit a gauge swatch in the round, it’s quite simple. Red is just a color that pops and makes people take notice. Having a red roof will definitely get people’s attention.
fixed widget title design for WooCommerce default widgets text and icon color in WooCommerce product rollover works correctly excerpt length is now followed by RSS feeds tweaked search results option for accuracy fixed woo carousel not saving more than 1 selected category corrected the name for 'avada_load_more_posts_name' filter mute video options now works correctly for Vimeo videos fixed youtube slider no preview image issue along with various other issues fixed blog timeline layout date issue where 2009/2010 posts were showing under the same heading fixed side header scrolling issue on iPad fusion slider will now prioritize to show the entire content on mobile mobile header background color now works when using a side header fixed content box heading color hover issue fixed animation settings for content boxes when parent settings were selected in the element fixed compatibility issue with optimize press fusion slider element heading / caption background color now works screen reader text is now properly hidden minor changes for WooCommerce quick checkout plugin to work correctly video preview image will now show up for Youtube/Vimeo videos on mobile/iPad fixed transparent header width in boxed mode tweaked strip HTML option for recent works mute options for videos now works for full width element ![]() new option to set the WordPress jpeg quality new fade option for rollover effects via theme options new dashboard widget for ThemeFusion related news and updates in the WP admin area ![]() new bbPress tab in theme options for better organization deprecated content-width options for layouts with sidebars, they are now auto-calculated using CSS calc() functions continued refactoring of the codebase for optimal performance and maintenance design cleanup for various bbPress areas and widgets added compatibility for WooCommerce 2.5 (Note: Woocommerce 2.5 is not released yet) added full compatibility for the filter bar add on for events calendar ![]() As a reminder, installment plans can help you pay down a larger bill, but you will continue accruing water consumption and additional monthly utility bill costs. 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The average monthly bill amount can vary each month, depending on the number of days in each billing cycle and future water use. Payment Arrangement and Bill Pay Options Average Monthly BillingĪverage monthly billing is a program to help smooth out seasonal highs and lows of your monthly utility bill. ![]() Oklahoma City, Oklahoma 73126-0570 By Phone Find an agent location near you at locations:. ![]() Use the MTCN number on the receipt to track the transaction. Western Union will provide a receipt to confirm your transaction. Western Union Agent Locationsįill out a form with the biller name (Oklahoma City Utilities), your account number, and the amount due. If there is change remaining, the amount will be credited to your Utilities account. The kiosks cannot give cash back, so be sure it is close to the amount owed when paying with cash. In January 2014, Intel reached an agreement to sell its OnCue service to. Kiosks are in all Homeland, Crest Foods, and 7-Eleven stores. 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